Detection of infectious myonecrosis virus (IMNV) of penaeid shrimp by reverse-transcriptase polymerase chain reaction (RT-PCR)
نویسندگان
چکیده
منابع مشابه
Reverse transcriptase-polymerase chain reaction (rt-PCR).
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...
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متن کاملPurification and characterization of infectious myonecrosis virus of penaeid shrimp.
The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named...
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متن کاملSimultaneous Detection of Infectious Hematopoietic Necrosis Virus (IHNV) and Infectious Pancreatic Necrosis Virus (IPNV) by Reverse Transcription (RT)-Polymerase Chain Reaction (PCR)
Multiple virus infections have recently been observed during routine examination of salmonid fish.1) Infectious hematopoietic necrosis virus (IHNV) and infectious pan creatic necrosis virus (IPNV) are often isolated together or with other viruses, such as a herpes virus or a retro-like virus.1,2) In case of multiple virus infections, only the virus which replicates most efficiently will be dete...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2006
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao073069